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Anna d'Este was born 16 November 1531 in Ferrara, Italy to Ercole II d'Este (1508-1559) and Renée of Orleans (1510-1574) and died 17 May 1607 Paris, France of unspecified causes. She married Francois de Guise (1519-1563) 29 April 1566 JL . She married Jacques de Savoie-Nemours (1531-1585) 5 May 1566 JL .
Children
| Name | Birth | Death | Joined with |
| Henri I de Guise (1550-1588) | |||
| Catherine de Guise (1552-1596) | |||
| Charles de Mayenne (1554-1611) | 26 March 1554 Alençon, Orne, Normandie, France | 3 October 1611 Soissons | Henriette de Savoie (1541-1611) |
| Louis II de Guise (1555-1588) | |||
| Antoine de Guise (1557-1560) | |||
| François de Guise (1559-1573) | |||
| Maximilien de Guise (1562-1567) | |||
| Name | Birth | Death | Joined with |
| Charles Emmanuel de Savoie (1567-1595) | |||
| Marguerite de Savoie (1569-1572) | |||
| Henri de Savoie (1572-1632) | 2 November 1572 Paris, France | 10 July 1632 Paris, France | Anne d'Aumale (1600-1638) |
