Barbara von Sachsen-Wittenberg was born circa 1405 to Rudolf III. von Sachsen-Wittenberg (c1367-1419) and Barbara von Liegnitz (-1435) and died 10 October 1465 Bayreuth, Germany of unspecified causes. She married Johann der Alchimist von Brandenburg-Kulmbach (1406-1464) 27 May 1416 JL .
Children
| Name | Birth | Death | Joined with |
| Barbara von Brandenburg (1423-1481) | 1423 | 7 November 1481 | Ludovico III Gonzaga (1412-1478) |
| Rudolf von Brandenburg-Kulmbach (1424-1424) | |||
| Elisabeth von Brandenburg (1425-1465) | |||
| Dorothea von Brandenburg (1430-1495) | 1430 | 10 November 1495 Kalundborg | Christopher III of Denmark (1416-1448) Christian I of Denmark (1426-1481) |