Isaac Bartlett was born 6 March 1726 in Marlborough, Middlesex County, Massachusetts to Daniel Bartlett (1691-1764) and Martha Howe (1686-1755) and died 11 March 1795 Holden, Worcester County, Massachusetts, United States of unspecified causes. He married Martha Warren (1728-1813) 1746 in Holden, Worcester County, Massachusetts.
Children
| Name | Birth | Death | Joined with |
| Patience Bartlett (1754-1813) | |||
| Lavina Bartlett (1756-) | |||
| Louisa Bartlett (1759-) | |||
| Eliada Bartlett (1761-1820) | |||
| Molly Bartlett (1762-1831) | |||
| Asahel Bartlett (1763-1831) | 13 August 1763 Holden, Worcester County, Massachusetts | 1831 | Hannah Fuller (1782-1832) |
| Daniel Bartlett (1765-) | |||
| Isaac Bartlett (1767-) | |||
| Percis Bartlett (1769-1791) | |||
| Levi Bartlett (1772-1818) | |||
| Artemas Bartlett (1774-1835) | |||
| Lydia Bartlett (1778-) | |||
Siblings
| Name | Birth | Death | Joined with |
| Jotham Bartlett (1717-1790) | |||
| Sarah Bartlett (1718-1795) | |||
| Daniel Bartlett (1719-1801) | |||
| Joseph Bartlett (1720-1790) | |||
| Abigail Bartlett (1721-1794) | |||
| Abraham Bartlett (1722-1723) | |||
| John Bartlett (1724-1764) | |||
| Jonathan Bartlett (1725-1781) | |||
| Isaac Bartlett (1726-1795) | 6 March 1726 Marlborough, Middlesex County, Massachusetts | 11 March 1795 Holden, Worcester County, Massachusetts, United States | Martha Warren (1728-1813) |
| Abner Bartlett (1727-) | |||
| Mary Bartlett (1728-) | |||
| Jonas Bartlett (1729-1808) | |||
| Mercy Bartlett (1730-1808) | |||