- Father: Louis Du Bois (1626-1696)
- Mother: Catherine Blanchan (1629-1706)
Married Maria Hasbrouck, who was born January 8, 1642/43 in Mutterstadt, Ludwigshafen, Rheinland-Pfalz, Germany. She died in 1718.
One daughter was named Mary Du Bois born 1684.