Isaac Little, Jr. was born 21 February 1677 in Marshfield, Plymouth County, Massachusetts to Isaac Little (1646-1699) and Bethiah Thomas (1650-1718) and died 2 February 1758 Pembroke, Plymouth County, Massachusetts of unspecified causes. He married Mary Otis (1685-1732) 1703 in Plymouth County, Massachusetts. He married Hannah Whitman (1709-1788) 10 January 1754 in Plympton, Plymouth County, Massachusetts.
Children
| Name | Birth | Death | Joined with |
| Mary Little (1704-) | 9 September 1704 Marshfield, Plymouth County, Massachusetts | Massachusetts | John Winslow (1703-1774) |
| Isaac Little (1710-) | |||
| Otis Little (1711-) | |||
| Mercy Little (1716-1723) | |||
| Nathaniel Little (1722-) | |||
Siblings
| Name | Birth | Death | Joined with |
| Thomas Little (1674-1712) | |||
| Dorothy Little (1676-1777) | |||
| Isaac Little (1677-1758) | 21 February 1677 Marshfield, Plymouth County, Massachusetts | 2 February 1758 Pembroke, Plymouth County, Massachusetts | Mary Otis (1685-1732) Hannah Whitman (1709-1788) |
| Bethiah Little (1681-1688) | |||
| Child Little (1683-1683) | |||
| Charles Little (1685-1724) | |||
| Abigail Little (1687-1769) | |||
| Bethiah Little (1688-1689) | |||
| Nathaniel Little (1690-1715) | |||
| Bethiah Little (1693-1751) | |||
| William Little (1692-) | |||
| Lemuel Little (1700-) | |||