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Isabella de Aviz de Portugal was born 24 October 1503 in Lisbon, Portugal to Manuel I of Portugal (1469-1521) and Maria of Aragon (1482-1517) and died 1 May 1539 Toledo, Spain of unspecified causes. She married Charles V of the Holy Roman Empire (1500-1558) 10 March 1526 JL in Seville, Spain.
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