James Ainslie was born 3 June 1808 in Fordyce, Aberdeenshire, Scotland, United Kingdom to Archibald Ainslie (-1827) and Euphemia Steedman (?-?) and died 24 June 1860 New Norfolk Hobart, Tasmania, Australia of unspecified causes. He married Mary Steven (1808-1887) 23 October 1825 in Hamilton, Lanarkshire, Scotland, United Kingdom.
He arrived in Hobart, Tasmania on 10 January 1853 on the Louisa with his wife and children.
Children
| Name | Birth | Death | Joined with |
| Archibald Ainslie (c1827-?) | |||
| George Ainslie (c1828-?) | |||
| Mary Stewart Ainslie (c1830-1925) | 1830 Scotland, United Kingdom | 1925 Hobart, Tasmania, Australia | Edwin Cox (c1825-1894) |
| Amily Annie Ainslie (c1831-1915) | 1831 Scotland, United Kingdom | December 1915 New Town, Tasmania, Australia | Francis Anderson (c1802-1875) |
| George Ainslie (c1833-?) | |||
| Euphemia Ainslie (c1835-?) | |||
| John Marshall Ainslie (c1837-?) | |||
| John Marshall Ainslie (c1839-?) | |||
| Jemima Ainslie (c1843-?) | |||
| Georgina Gordon Ainslie (c1845-1929) | 1845 Scotland, United Kingdom | 15 July 1929 Grafton, New South Wales, Australia | Alexander Fairweather (1849-1919) |
| Jemima Ainslie (c1848-?) | |||
Footnotes (including sources)
| ‡ General |