James Gleason was born circa 1684 in Sudbury, Middlesex County, Massachusetts to John Gleason (1647-1689) and Mary Ross (1656-1688) and died circa 1750 Marlborough, Middlesex County, Massachusetts of unspecified causes. He married Mary Barrett (1695-1768) 24 November 1713 in Marlborough, Middlesex County, Massachusetts.
Children
| Name | Birth | Death | Joined with |
| Daniel Gleason (1715-1715) | |||
| Mary Gleason (1716-) | |||
| Sarah Gleason (1718-) | |||
| Abigail Gleason (1722-) | |||
| John Gleason (1724-1816) | |||
| Martha Gleason (1724-1793) | |||
| Joseph Gleason (1726-1805) | 1726 Sudbury, Middlesex County, Massachusetts | 14 May 1805 Marlborough, Middlesex County, Massachusetts | Persis Allen (1736-1812) |
| Zaccheus Gleason (1728-1730) | |||
| Submit Gleason (1733-) | |||
Siblings
| Name | Birth | Death | Joined with |
| Mary Gleason (1681-) | |||
| James Gleason (1684-1750) | 1684 Sudbury, Middlesex County, Massachusetts | 1750 Marlborough, Middlesex County, Massachusetts | Mary Barrett (1695-1768) |
| Thomas Gleason (1686-1755) | |||
| Martha Gleason (1688-) | |||