John Barlow was born circa 1715 in Maryland, United States of America to James Barlow (c1680-1721) and Johanna (c1682-) and died 1788 Anne Arundel County, Maryland, United States of America of unspecified causes. He married Elizabeth Keith (1717-1783) 12 February 1736 in Christ Church, Queen Caroline Parrish, Anne Arundel County, Maryland, United States.
Children
| Name | Birth | Death | Joined with |
| Barsheba Barlow (c1740-) | 1740 Anne Arundel County, Maryland, United States of America | Joshua Kirby | |
| Prudence Barlow (c1744-) | 1744 Anne Arundel County, Maryland, United States of America | Joseph Lowe. | |
| Temperance Barlow (c1750-1821) | 1750 Anne Arundel County, Maryland, United States of America | 1821 Deaverton, Morgan County, Ohio, United States of America | Peter Burgoon (c1750-c1821) |
| Susannah Barlow (1755-1797) | 1755 Elkbridge, Anne Arundel County, Maryland, United States of America | 1797 Cambria County, Pennsylvania, United States of America | John Burgoon (1745-1797) |
Siblings
| Name | Birth | Death | Joined with |
| John Barlow (1715-1788) | 1715 Maryland, United States of America | 1788 Anne Arundel County, Maryland, United States of America | Elizabeth Keith (1717-1783) |
| James Barlow | |||
| Joab Barlow | |||
| Bathsheba Barlow | |||
| Zachariah Barlow | |||
| William Barlow | |||