John Mayo was born 17 July 1737 in Gloucester Courthouse, Virginia, United States to William Mayo (1684-1744) and Ann Perratt (1701-1773) and died 13 January 1786 Richmond, Virginia, United States of unspecified causes.
Children
| Name | Birth | Death | Joined with |
| William Mayo (1757-1837) | 25 September 1757 Gloucester Courthouse, Virginia, United States | 12 August 1837 Richmond, Virginia, United States | Elizabeth Bland Poythress (1759-1806) |
Siblings
| Name | Birth | Death | Joined with |
| John Mayo (1737-1786) | 17 July 1737 Gloucester Courthouse, Virginia, United States | 13 January 1786 Richmond, Virginia, United States | Mary Tabb (1733-1792) |