Margaret Lyon was born 11 December 1547 in Glamis, Angus, Scotland, United Kingdom to John Lyon, 7th Lord Glamis (1521-1558) and Janet Keith (-1559) and died December 1626 Avondale, Lanarkshire, Scotland, United Kingdom of unspecified causes. She married Gilbert Kennedy, 4th Earl of Cassilis (1541-1576) 30 September 1566 JL . She married John Hamilton, 1st Marquess of Hamilton (c1535-1604) 30 December 1577 JL . Robert I of Scotland (1274-1329)/s.
|Offspring of Margaret Lyon and Gilbert Kennedy, 4th Earl of Cassilis (1541-1576)|
|John Kennedy, 5th Earl of Cassilis (1575-1615)|
|Hew Kennedy (c1577-1607)|
|Offspring of Margaret Lyon and John Hamilton, 1st Marquess of Hamilton (c1535-1604)|
|James Hamilton, 2nd Marquess of Hamilton (1589-1625)||1589 Isle of Arran, North Ayrshire, Scotland, United Kingdom||2 March 1625 Whitehall, Greater London, England, United Kingdom||Anne Cuninghame (bef1588-1647) Anne Cuninghame (bef1588-1647) Anne Stewart (-aft1669)|
|Margaret Hamilton (-aft1606)|
|Edward Hamilton (bef1589-bef1604)|