Samuel Knowles I was born 17 September 1651 in Plymouth, Plymouth County, Massachusetts to Richard Knowles (1614-1682) and Ruth Bower (1616-1687) and died 19 June 1737 Eastham, Barnstable County, Massachusetts of unspecified causes. He married Mercy Freeman (1659-1744) December 1679 in Eastham, Barnstable County, Massachusetts.
Gravestone
Gravestone for Samuel Knowles
Gravestone Inscription:
HERE LYES THE BODY OF MR SAMUEL KNOWLES FORMERLY REPRESENTATIVE FOR THE TOWN OF EASTHAM WHO DIED JUNE Y 19 1737 IN THE 85 YEAR OF HIS AGE
Location of Burial: Orleans Cemetery East Orleans MA
Children
| Name | Birth | Death | Joined with |
| James Knowles (1680-1704) | |||
| Samuel Knowles (1683-1750) | |||
| Nathaniel Knowles (1686-1732) | |||
| Richard Knowles (1688-1769) | 28 July 1688 Eastham, Barnstable County, Massachusetts | 1769 Chatham, Barnstable County, Massachusetts | Martha Cobb (1682-1763) |
| Rebecca Knowles (1690-1754) | |||
| John Knowles (1692-1754) | |||
| Ruth Knowles (1694-1745) | |||
| Cornelius Knowles (1695-1764) | |||
| Amos Knowles (1702-1786) | |||
| James Knowles (1705-1765) | |||
Siblings
| Name | Birth | Death | Joined with |
| John Knowles (1633-1705) | 11 March 1633 Glouceby, Lincolnshire, England | 5 December 1705 Hampton, Rockingham County, New Hampshire | Jemima Austin (1641-1705) Apphia Bangs (1651-1722) |
| Mary Knowles (1642-1692) | |||
| James Knowles (1648-1678) | |||
| Samuel Knowles (1651-1737) | 17 September 1651 Plymouth, Plymouth County, Massachusetts | 19 June 1737 Eastham, Barnstable County, Massachusetts | Mercy Freeman (1659-1744) |
| Mehitable Knowles (1653-1721) | |||
| Barbara Knowles (1656-1715) | 28 September 1656 Eastham, Barnstable County, Massachusetts | 23 February 1715 Eastham, Barnstable County, Massachusetts | Thomas Mayo (1651-1729) |
| Ruth Duty Knowles (1657-1714) | 3 November 1657 Plymouth, Plymouth County, Massachusetts | 28 August 1714 Eastham, Barnstable County, Massachusetts | Joseph Collins (1645-1724) |