The Test Resolution tool in Borland Genetics allows user to measure the resolution of synthetic DNA files.
No-Calls[]
A no-call is a row of a raw DNA file that contains no data. In factory kits from the major testing companies, typically about 1% - 2% of the SNPs tested yield no-calls due to experimental error, resulting in a 98%-99% resolution as measured across the template. Phased data contains many more no-calls due to the inability of some rows of data to be unambiguously phased between donors. Therefore, while phased and/or the more general class of Mono kits are required for reconstructive analysis, the resolution of a Mono kit will rarely reach above 85%, even when data is imputed to fill no-calls by comparing redundant DNA strands from a variety of donors. This is well above the required resolution to produce accurate cousin lists on sites such as GedMatch that allow the upload of synthetic kits.
Scope of Tool[]
The Test Resolution tool in Borland Genetics self-selects the scope of the resolution measurement depending on whether the kit is full or partial, mono or stereo, so as to provide meaningful results as to the quality of the reconstruction not skewed by filler data used in gaps between reconstructed segments.
Notes Regarding Uploading to Classic GEDmatch[]
Note that the final merge from Mono to Stereo of a reconstructed kit’s component clades also introduces additional no-calls into the reconstruction in order to ensure GedMatch compatibility of the resultant kit. This type of no-call, however, generally does NOT decrease the effective resolution when uploaded to the GedMatch template, and therefore does not impair the ability to accurately generate cousin lists on sites such as GedMatch that allow the upload of synthetic kits.