William Stuart Appleton was born 1 June 1814 in Cambridge, Middlesex County, Massachusetts to Ebenezer Appleton (1784-1833) and Sarah Patterson (1788-1837) and died 7 March 1891 of unspecified causes. He married Georgiana Louisa Frances Armistead (1817-) 27 November 1838 in Baltimore, Baltimore County, Maryland.
Children
| Name | Birth | Death | Joined with |
| Louisa Armistead Appleton (1839-1901) | |||
| Sarah Patterson Appleton (1840-1841) | |||
| William Stuart Appleton (1841-1845) | |||
| George Armistead Appleton (1843-1877) | |||
| Ebenezer Appleton (1845-1925) | |||
| Georgiana Louisa Frances Armistead Appleton (1847-) | |||
| Edith Ida Stuart Appleton (1849-1892) | 11 June 1849 Baltimore, Baltimore County, Maryland | 20 January 1892 Boston, Suffolk County, Massachusetts | William Sumner Appleton (1840-1903) |
| Margaret Armitta Appleton (1851-) | |||
| Caroline Frances Appleton (1853-1857) | |||
| Alice Maud Appleton (1859-1893) | |||
Siblings
| Name | Birth | Death | Joined with |
| Sarah Appleton (1810-1837) | |||
| Samuel Appleton (1811-1861) | |||
| William Stuart Appleton (1814-1891) | 1 June 1814 Cambridge, Middlesex County, Massachusetts | 7 March 1891 | Georgiana Louisa Frances Armistead (1817-) |
| Caroline Frances Appleton (1817-1901) | |||